Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 1

Research Article

SIRT7 Regulates Lipopolysaccharide-Induced Inflammatory Injury by Suppressing the NF-κB Signaling Pathway

Figure 1

SIRT7 expression in tissues from mastitic cattle and LPS-treated DCMECs. (a) SIRT7 expression was reduced in five different samples from mastitic cattle. (b) DCMECs were cultured in vitro and identified using antibodies specific to cytokeratin-18 via immunofluorescence staining. The white arrows indicate the specific localization of cytokeratin-18. Blue: DNA; green: cytokeratin. (c, d) qRT-PCR was carried out to evaluate SIRT7 expression in DCMECs treated with varying concentrations of LPS (1 μg/mL LPS in (c); 10 μg/mL LPS in (d)) at different time points. (e) SIRT7 expression in DCMECs at different time points was evaluated via western blotting after LPS (1 μg/mL LPS) treatment of DCMECs. (f) Quantification of SIRT7 protein levels in DCMECs using ImageJ after LPS (1 μg/mL LPS) treatment of DCMECs. Data are expressed as and .

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