Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 1

Research Article

SIRT7 Regulates Lipopolysaccharide-Induced Inflammatory Injury by Suppressing the NF-κB Signaling Pathway

Figure 1

SIRT7 expression in tissues from mastitic cattle and LPS-treated DCMECs. (a) SIRT7 expression was reduced in five different samples from mastitic cattle. (b) DCMECs were cultured in vitro and identified using antibodies specific to cytokeratin-18 via immunofluorescence staining. The white arrows indicate the specific localization of cytokeratin-18. Blue: DNA; green: cytokeratin. (c, d) qRT-PCR was carried out to evaluate SIRT7 expression in DCMECs treated with varying concentrations of LPS (1 μg/mL LPS in (c); 10 μg/mL LPS in (d)) at different time points. (e) SIRT7 expression in DCMECs at different time points was evaluated via western blotting after LPS (1 μg/mL LPS) treatment of DCMECs. (f) Quantification of SIRT7 protein levels in DCMECs using ImageJ after LPS (1 μg/mL LPS) treatment of DCMECs. Data are expressed as and .
(a)
(b)
(c)
(d)
(e)
(f)

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.