Histone Acetyltransferase-Dependent Pathways Mediate Upregulation of NADPH Oxidase 5 in Human Macrophages under Inflammatory Conditions: A Potential Mechanism of Reactive Oxygen Species Overproduction in Atherosclerosis

Figure 1

Human atherosclerotic plaques exhibit a significant upregulation of p300, HAT1, H3K27ac, and Nox5 proteins. Note the densitometric analysis of type A-HAT (p300) (a), type B-HAT (HAT1) (b), H3K27ac (e), and Nox5 (f) protein levels analyzed by western blot of atherosclerotic (carotid artery) and nonatherosclerotic (superior thyroid artery) tissues derived from patients undergoing extended endarterectomy. ; , . values were taken in relation to nonatherosclerotic condition. Representative immunoblots depicting the induction of p300 (c), HAT1 (d), H3K27ac (g), and Nox5 (h) proteins in atherosclerotic carotid arteries. Non-athero: nonatherosclerotic tissue; Athero: atherosclerotic tissue.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)