Protocatechuic Acid-Mediated miR-219a-5p Activation Inhibits the p66shc Oxidant Pathway to Alleviate Alcoholic Liver Injury

Figure 3

PCA reduced p66shc-mediated ROS formation in ethanol-exposed AML-12 cells. AML-12 cells were transfected with p66shc-specific siRNA or control siRNA for 36 h, 10 μM PCA for 6 h, and/or 100 mM ethanol for 24 h. (a, b) Protein levels of p66shc and MnSOD (); (c) immunofluorescence staining for the p66shc antibody in AML-12 cells for proliferation analysis. . (d) Hepatic CAT levels (), (e) hepatic GSH levels (), (f) hepatic MDA levels (), and (g) hepatic H₂O₂ levels (). (h) DCFH-DA staining. . and compared with the control group; ^## and ^# compared with the ethanol group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)