Research Article
Protocatechuic Acid-Mediated miR-219a-5p Activation Inhibits the p66shc Oxidant Pathway to Alleviate Alcoholic Liver Injury
Figure 5
miR-219a-5p attenuated p66shc-mediated ROS formation in vitro. (a–c) AML-12 cells were cotransfected with ago-219a, si-p66shc, or the corresponding negative control, as indicated. (a) qRT-PCR analysis of the miR-219a-5p expression (). (b, c) Protein levels of p66shc and MnSOD (). (d–f) AML-12 cells were cotransfected with ant-219a, si-p66shc, or the corresponding negative control, as indicated. (d) qRT-PCR analysis of the miR-219a-5p expression (). (e, f) Protein levels of p66shc and MnSOD (). and compared with the control group; ## and # compared with the ethanol group; && and & compared with cells transfected with ant-219 with ethanol.
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