Research Article
Inhibition of Mitofusin-2 Promotes Cardiac Fibroblast Activation via the PERK/ATF4 Pathway and Reactive Oxygen Species
Table 2
Primer sequences and amplicon sizes for real-time RT-PCR.
| | Genes | Primer sequence (5-3) |
| | COL-1 | F: 5ACGTCCTGGTGAAGTTGGTC3
R: 5TCCAGCAATACCCTGAGGTC3 | | CTGF | F: 5CAGGGAGTAAGGGACACGA3
R: 5ACAGCAGTTAGGAACCCAGAT3 | | α-SMA | F: 5CCGAGATCTCACCGACTACC3
R: 5ATGCCACAGGATTCCATACCC3 | | Mfn2 | F: 5CACTTGTCTTCCCCAATGAG3
R: 5GGAGAGGAAACCUGAC3 | | β-Actin | F: 5CCUCUCCUUUGGACUGUAU3
R: 5ATGCCACAGGATTCCATACCC3 | | TGF-β1 | F: 5TGAGTGGCTGTCTTTTGACG3
R: 5ACTGAAGCGAAAGCCCTGTA3 | | ATF4 | F: 5TGGAGUGACCGCUCACAUUU3
R: 5CCUCACUGGCGAGUGUAAA3 |
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