Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 6

Research Article

Bloom Syndrome Protein Activates AKT and PRAS40 in Prostate Cancer Cells

Figure 6

BEZ235 and GDC-0068 reduce proliferation and induce apoptosis more potently than CDDP in PC cells. (a) Inhibition ratios in prostate cancer cells following 48 h treatment: for PC-3 cells, BEZ235 IC50— nmol/L, CDDP IC50— nmol/L, and GDC-0068 IC50— nmol/L; for LnCAP cells, BEZ235 IC50— nmol/L, CDDP IC50— nmol/L, and GDC-0068 IC50— nmol/L; and for VCAP cells, BEZ235 IC50— nmol/L, CDDP IC50— nmol/L, and GDC-0068 IC50— nmol/L (). (b) Apoptosis ratios in various prostate cancer cell lines after drug treatments. At a concentration of 0.5 μmol/L, BEZ235 and GDC-0068 induced apoptosis in ~40% of cancer cells. CDDP induced lower relative levels of apoptosis (~20%; 24 h treatment). (c) Cells were treated with BEZ235 (2 μmol/L), GDC-0068 (2 μmol/L), and CDDP (10 μmol/L) for 24 and 48 hours. GDC-0068, BEZ235, and CDDP significantly increased ROS production in tumor cells. Values represent the deviation (SD); and .
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