Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 7

Research Article

Bloom Syndrome Protein Activates AKT and PRAS40 in Prostate Cancer Cells

Figure 7

BLM, pAKT, and pPRAS40 expression in PC-3 cells. (a) BLM helicase, pAKT, and pPRAS40 expression in PC-3 cells as measured by western blot and WES analysis. (b) Cells treated with BEZ235 and GDC-0068 displayed significantly reduced BLM expression, while BLM knockout decreased pAKT (Ser473) and pPRAS40 (Thr246) levels. BLM expression values were normalized to β-actin, and pAKT and pPRAS40 (Thr246) expression values were normalized to total AKT1 and PRAS40, respectively. Data represent the ( and ). BLM expression is positively correlated with (c) pAKT () and (d) pPRAS40 levels (). (e) ROS production is negatively correlated with BLM expression ().

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