Nrg1 Intracellular Signaling Is Neuroprotective upon Stroke

Figure 4

OGD stimulates Nrg1 intracellular signaling in vitro. (a) Representative images of neurons expressing Nrg1 tagged with GFP in its C-terminal domain. (a–c) represent the normoxic control, and (d–o) depict images of neurons after hypoxia. OGD was performed at D14, and the neurons were fixed immediately after 30 or 90 minutes of OGD or after 60 minutes of recovery after OGD. Treatment with 40 mM of KCl for 1 hour was used as a positive control. Scale bar, 10 μm. (p) The graph illustrates the ratio of the quantification of GFP fluorescence intensity in the cell nucleus (Nrg1-ICD) to that in the soma (Nrg1-FL). . . , , one-way ANOVA, and Tukey’s multiple comparisons test. .