GSSG/GSH as indirect measure of glutathione peroxidase activity
60 male C57BL/6 wild-type mice divided into 3 I/R groups (60 min of ischemia, 90 min reperfusion) and 3 corresponding sham groups Baicalein administered intraperitoneally 30 minutes before laparotomy; controls received an equal volume of either the vehicle (DMSO) or saline
Pretreatment with Baicalein increased intrahepatic glutathione peroxidase activity significantly; sole application of DMSO also elevated the activity level, although not statistically significant
OS occurred in db/db mice immediately after PH, peaked at 30 minutes, and remained elevated for at least 8 hours; a significant difference between controls and db/db mice was shown
25 male Sprague-Dawley rats Sham mobilization but no hepatectomy; standard hepatectomy without prior mobilization; protocol like sham with following hepatectomy; clamping of the entire hepatic pedicle ( ischemia, 5 min reperfusion) with following hepatectomy; lobes to be resected were mobilized and clamped ( ischemia, 5 min reperfusion) with following hepatectomy
MDA: after hepatectomy ↑ in PH, NCPH, TCPH, and RPH compared with the sham group; , all with peaks at 24 h; RPH also increased but without a peak and with a faster decrease SOD and catalase: levels decreased in all groups compared to the sham group; the lowest levels are found in TCPH and the highest levels in RPH
Gclm and Gclm (wild-type = control) mice 2/3 PH was performed on 8-10-week-old males
Baseline GSH levels of Gclm mice are about 15% of wt mice and Gclm mice maintained low GSH levels after 2/3 PH, whereas wt mice showed a 2-fold increase of GSH levels during the early phase of regeneration Gclm mice showed a delayed peak in cell proliferation compared to wt mice