Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 5

Research Article

Upregulation of Transient Receptor Potential Canonical Type 3 Channel via AT1R/TGF-β1/Smad2/3 Induces Atrial Fibrosis in Aging and Spontaneously Hypertensive Rats

Figure 5

Intracellular Ca2+ rises induced by Ang II with or without extracellular Ca2+. Intracellular free Ca2+ detected by ratio values of Fura340/380. During the fluorescence calcium acquisition process, in the control group, fibroblasts were perfused with Ca2+-free Tyrode’s solution for 1 minute, following perfusion with Ca2+-free Tyrode’s solution with Ang II (1 μmol/L) for 4 minutes, and then perfused with 1.8 mmol/L Ca2+ Tyrode’s solution with Ang II (1 μmol/L) for 4 minutes. In the Pyr3 group, fibroblasts were perfused with Ca2+-free Tyrode’s solution with Pyr3 (10 μmol/L) for 1 minute, following perfusion with Ca2+-free Tyrode’s solution with Ang II (1 μmol/L) for 4 minutes containing Pyr3 (10 μmol/L), and then perfused with 1.8 mmol/L Ca2+ Tyrode’s solution with Ang II (1 μmol/L) for 4 minutes containing Pyr3 (10 μmol/L). (a) The changes of fluorescence calcium microphotographs, traces, and quantitative ratio values. (b) The statistical graph of fluorescence calcium ratio values. Baseline bar shows the mean values of Ca2+-free Tyrode’s solution, Ang II bar shows the peak values of calcium transient of Ca2+-free Tyrode’s solution with Ang II (1 μmol/L), and Ang II+Ca2+ bar shows the peak values of calcium transient with 1.8 mmol/L Ca2+ Tyrode’s solution with Ang II (1 μmol/L).
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