Research Article
Alpha Lipoic Acid Improves Endothelial Function and Oxidative Stress in Mice Exposed to Chronic Intermittent Hypoxia
Figure 4
Uncoupled endothelial NOS in the endothelium. Representative images of dihydroethidium (DHE) staining of the endothelial monolayer before and after incubation with the endothelial NOS inhibitor L-NAME (20x magnification) (a). Quantification of fluorescence levels using corrected total cell florescence (CTCF) (b). Values are displayed as from 10 mice. Statistical analysis was done by using Student’s -test within groups before and after L-NAME. Two-way repeated measures ANOVA followed by Bonferroni post-hoc test was used for comparison between groups after L-NAME. before vs. after L-NAME, versus IARD, versus IALA, and versus IHRD. CTCF: corrected total cell fluorescence; DHE: dihydroethidium; L-NAME: Nω-nitro-L-arginine methyl ester; NOS: nitric oxide synthase.
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