Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 7

Research Article

Zerumbone Exhibits Antiphotoaging and Dermatoprotective Properties in Ultraviolet A-Irradiated Human Skin Fibroblast Cells via the Activation of Nrf2/ARE Defensive Pathway

Figure 7

ZER-mediated protective effect against UVA radiation was attenuated in Nrf2 knockdown HSF cells. (a, b) Cells were transfected with specific siRNA against Nrf2 or a nonsilencing control, followed by treatment with ZER (8 μM, 2 h), and the expression patterns of total Nrf2 and HO-1 proteins in both Nrf2-transfected and nonsilencing control were measured using western blot method. (c, d) In ZER-pretreated (8 μM, 24 h) and UVA-exposed HSF cells, the effect of Nrf2 silencing on intracellular ROS accumulation was measured using DCFH2-DA (10 μM) fluorescence staining method. Data were presented as fold change of ROS in nonsilencing control and Nrf2 knockdown cells. Results were presented as of three or more assays. , , compared to untreated control cells. #, ##, compared to ZER-treated cells.

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