Zerumbone Exhibits Antiphotoaging and Dermatoprotective Properties in Ultraviolet A-Irradiated Human Skin Fibroblast Cells via the Activation of Nrf2/ARE Defensive Pathway
ZER-mediated protective effect against UVA radiation was attenuated in Nrf2 knockdown HSF cells. (a, b) Cells were transfected with specific siRNA against Nrf2 or a nonsilencing control, followed by treatment with ZER (8 μM, 2 h), and the expression patterns of total Nrf2 and HO-1 proteins in both Nrf2-transfected and nonsilencing control were measured using western blot method. (c, d) In ZER-pretreated (8 μM, 24 h) and UVA-exposed HSF cells, the effect of Nrf2 silencing on intracellular ROS accumulation was measured using DCFH2-DA (10 μM) fluorescence staining method. Data were presented as fold change of ROS in nonsilencing control and Nrf2 knockdown cells. Results were presented as of three or more assays. ,, compared to untreated control cells. #,##, compared to ZER-treated cells.