Chloramphenicol Mitigates Oxidative Stress by Inhibiting Translation of Mitochondrial Complex I in Dopaminergic Neurons of Toxin-Induced Parkinson’s Disease Model

Figure 3

CP decreases mitochondrial function in SN4741 cells by reducing the amount of mitochondrial complex I. (a–c) Oxygen consumption rate (OCR), a direct indicator of mitochondrial function, was measured using an XF24 analyzer after treating SN4741 cells with 2.5 or 10 μg/ml CP for 24 h. (a) Measurement of changes in OCR (). (b) Bar graph showing basal OCR (). (c) Bar graph showing proton leak (). (d) Activity of mitochondrial complex I-V isolated from SN4741 cells, determined by enzymatic assay (). (e) The amount of mRNA for mitochondrial complex I–V subunits, determined by qPCR (). (f) Expression levels of mitochondrial complex I–V subunit proteins were measured by Western blotting (). (g) Bar graph showing quantification of mitochondrial complex I-V subunit expression, measured by Western blotting (). (h) Expression levels of mitochondrial supercomplex I, II proteins were measured by BN-PAGE () (i) A bar graph () showing quantification of mitochondrial supercomplex protein expression. All data are representative of three independent experiments. by one-way ANOVA in (a–c) and by 2-tailed unpaired Student’s -test in (d, e, g, i); ns: not significant. Error bars represent +SD.

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