Research Article
Glycine Protects against Hypoxic-Ischemic Brain Injury by Regulating Mitochondria-Mediated Autophagy via the AMPK Pathway
Figure 5
Glycine attenuated CoCl2-induced cytotoxicity, cellular ROS, and apoptosis in PC12 cells. (a) PC12 cells treated with CoCl2 on dose-dependent manner for 12 hours and 24 hours by CCK8. (b) PC12 cells were pretreated with different concentrations of glycine for 12 hours and 24 hours. Then, PC12 cells were treated with CoCl2 (800–1000 μM) and cell viability was determined by CCK8. , , and versus the control group. ## and ### versus the CoCl2 group. (c) ROS generation of the control group, CoCl2 group, and CoCl2 + glycine group was determined by flow cytometry assay of DCFH-DA. . . (d) Apoptotic cells of each group were detected by TUNEL and DAPI staining. . .
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