Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 1

Research Article

NANOG Attenuates Hair Follicle-Derived Mesenchymal Stem Cell Senescence by Upregulating PBX1 and Activating AKT Signaling

Figure 1

Isolation and identification of hair follicle-derived mesenchymal stem cells (HF-MSCs). (a, b) Cells that migrated from hair follicles exhibited fibroblast-like characteristics. Scale . (c) Immunofluorescence assay showing that the cells expressed CD44, CD73, CD90, and CD105 but not CD31. (d) Flow cytometry was used to measure the cell surface expression of MSC markers. (e) HF-MSCs were cultured in osteogenic differentiation media and stained (red) with Alizarin Red S. Scale . (f) HF-MSCs were cultured in adipogenic differentiation media and stained (red) with Oil Red O. Scale .