Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 3

Research Article

NANOG Attenuates Hair Follicle-Derived Mesenchymal Stem Cell Senescence by Upregulating PBX1 and Activating AKT Signaling

Figure 3

Ectopic NANOG expression and PBX1 overexpression regulate osteogenesis and adipogenesis, respectively. (a, b) NANOG and PBX1 promoted osteogenesis based on Alizarin Red S staining and quantitative detection. Scale . (c, d) NANOG and PBX1 repressed adipogenesis based on Oil Red O staining and quantitative detection. Scale . (e, f) Blue cells were considered to be positive for senescence based on senescence-associated β-galactosidase (SA-β-gal) staining. There were fewer positive cells in NANOG- and PBX1-expressing groups in comparison to the vector control group at both passages 6 and 14. Scale . (g) Western blotting showed that PBX1 expression decreased and senescence-related protein expression increased with increasing passages (, , ).