NANOG Attenuates Hair Follicle-Derived Mesenchymal Stem Cell Senescence by Upregulating PBX1 and Activating AKT Signaling
Ectopic NANOG expression and PBX1 overexpression regulate osteogenesis and adipogenesis, respectively. (a, b) NANOG and PBX1 promoted osteogenesis based on Alizarin Red S staining and quantitative detection. Scale . (c, d) NANOG and PBX1 repressed adipogenesis based on Oil Red O staining and quantitative detection. Scale . (e, f) Blue cells were considered to be positive for senescence based on senescence-associated β-galactosidase (SA-β-gal) staining. There were fewer positive cells in NANOG- and PBX1-expressing groups in comparison to the vector control group at both passages 6 and 14. Scale . (g) Western blotting showed that PBX1 expression decreased and senescence-related protein expression increased with increasing passages (,,).