Efficiently Capturing Mitochondria-Targeted Constituents with Hepatoprotective Activity from Medicinal Herbs

Figure 4

Determination of a mixed standard solution using CM-HMC. (a) HPLC chromatograms of a mixed standard solution are shown for ultrafiltrates derived from active HM (black line) and denatured HM (red line), which served as a control. Peaks R2 and R3 exhibited remarkable area enhancement compared with the control. However, the area of the R1 peak was equal to that of the control. (b) HPLC chromatogram of a mixed standard solution, which was directly assayed by LC/MS. GL: glucuronide; AC: amoxicillin; DZ: daidzin; and SB: silybin.

(a)

(b)