Cucurbitacin B Exerts Antiaging Effects in Yeast by Regulating Autophagy and Oxidative Stress

Table 1

Yeast strains used in the present study.

Strains	Genotype	Source
K6001	MATa, ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, GAL, psi+, ho::HO::CDC6 (at HO), cdc6::hisG, ura3::URA3 GAL-ubiR-CDC6 (at URA3)	Gifted by Professor Michael Breitenbach
Δuth1 of K6001, Δskn7 of K6001, Δsod1 of K6001, Δsod2 of K6001, Δatg2 of K6001, Δatg32 of K6001	Replace the UTH1 gene, SKN7 gene, SOD1 gene, SOD2 gene, ATG2 gene, and ATG32 gene in K6001 with kanamycin gene, respectively	Constructed by Professor Akira Matsuura
BY4741	MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0	Gifted by Professor Akira Matsuura
YOM36	Prototrophic derivative of BY4742 (MATα, his3Δ1, leu2Δ0, lys2Δ0, ura3Δ0)	Gifted by Professor Akira Matsuura
Δatg2 of YOM36, Δatg32 of YOM36	Replace the ATG2 gene and ATG32 gene in YOM36 with kanamycin gene, respectively	Constructed by Professor Akira Matsuura
YOM38 containing plasmid pR316-GFP-ATG8	Prototrophic derivative of BY4742 (MATα, his3Δ1, leu2Δ0, lys2Δ0) containing plasmid pR316-GFP-ATG8	Constructed by Professor Akira Matsuura
YO2458	MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0, YOR202w::KanMX4	Provided by Professor Yoshikazu Ohya