Oxidative Medicine and Cellular Longevity / 2019 / Article / Tab 1

Research Article

Cucurbitacin B Exerts Antiaging Effects in Yeast by Regulating Autophagy and Oxidative Stress

Table 1

Yeast strains used in the present study.

StrainsGenotypeSource

K6001MATa, ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, GAL, psi+, ho::HO::CDC6 (at HO), cdc6::hisG, ura3::URA3 GAL-ubiR-CDC6 (at URA3)Gifted by Professor Michael Breitenbach
Δuth1 of K6001, Δskn7 of K6001, Δsod1 of K6001, Δsod2 of K6001, Δatg2 of K6001, Δatg32 of K6001Replace the UTH1 gene, SKN7 gene, SOD1 gene, SOD2 gene, ATG2 gene, and ATG32 gene in K6001 with kanamycin gene, respectivelyConstructed by Professor Akira Matsuura
BY4741MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0Gifted by Professor Akira Matsuura
YOM36Prototrophic derivative of BY4742 (MATα, his3Δ1, leu2Δ0, lys2Δ0, ura3Δ0)Gifted by Professor Akira Matsuura
Δatg2 of YOM36, Δatg32 of YOM36Replace the ATG2 gene and ATG32 gene in YOM36 with kanamycin gene, respectivelyConstructed by Professor Akira Matsuura
YOM38 containing plasmid pR316-GFP-ATG8Prototrophic derivative of BY4742 (MATα, his3Δ1, leu2Δ0, lys2Δ0) containing plasmid pR316-GFP-ATG8Constructed by Professor Akira Matsuura
YO2458MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0, YOR202w::KanMX4Provided by Professor Yoshikazu Ohya

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