Adaptations in Protein Expression and Regulated Activity of Pyruvate Dehydrogenase Multienzyme Complex in Human Systolic Heart Failure
(a) Relative PDK4 levels (ng/mg total myocardial protein) in nonfailing () and heart failure () groups measured by ELISA assay ( vs nonfailing). (b) Western immunoblot band examples for PDK1 and PDK2 expression in independent gel assays. Nonfailing and heart failure groups measured concurrently in each gel. (c) Summary of PDK1 and PDK2 protein levels from Western immunoblot assays normalized to porin (VDAC). Myocardial porin protein expression did not differ significantly between groups.