Effects of the (Pro)renin Receptor on Cardiac Remodeling and Function in a Rat Alcoholic Cardiomyopathy Model via the PRR-ERK1/2-NOX4 Pathway

Figure 4

Oxidative stress; protein expression levels of PRR, NOX4, and TGF-β; and phosphorylation of ERK1/2 after PRR gene transfer in primary neonatal cardiac fibroblasts (CFs). (a) Representative dihydroethidium (DHE) fluorescence image of CFs (scale ). (b, c) Statistical results of oxidative stress by DHE relative fluorescence and NADPH oxidase activity. (d) Representative western blot analysis of ERK1/2 phosphorylation and PRR, NOX4, and TGF-β protein expression. (e)–(h) Quantification of protein expression levels of PRR/GAPDH, NOX4/GAPDH, p-ERK1/2/t-ERK1/2, and TGF-β/GAPDH. and versus the control group, versus the EGFP group, and versus the PRR group. Tel: telmisartan.

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