Hypoxia-Induced ROS Contribute to Myoblast Pyroptosis during Obstructive Sleep Apnea via the NF-κB/HIF-1α Signaling Pathway

Figure 5

ROS mediate hypoxia-induced pyroptosis in myoblasts. (a) Pellets of C2C12 cells after treatment with or without 2 mM NAC under normoxic control and CoCl₂-induced hypoxic conditions for 24 hours. (b, c) Flow cytometry analysis of the relative cell size (FSC) and cell complexity (SSC) of C2C12 cells treated with or without NAC under normoxic and CoCl₂-induced hypoxic conditions for 24 hours (). (d, e) ROS assay in C2C12 cells treated with or without NAC under normoxic and CoCl₂-induced hypoxic conditions for 24 hours. Scale  μm. (f) NAC treatment decreased the level of LDH release induced by CoCl₂ in C2C12 cells (). (g) Real-time PCR analysis of relative inflammatory gene expression in C2C12 myoblasts treated with or without NAC for 8 hours (). (h–j) Western blot analysis of pyroptosis-related protein expression in C2C12 cells treated with or without NAC under normoxic control and CoCl₂-induced hypoxic conditions for 24 hours. The data are shown as the . , , ; NS: no significant difference.

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