Hypoxia-Induced ROS Contribute to Myoblast Pyroptosis during Obstructive Sleep Apnea via the NF-κB/HIF-1α Signaling Pathway

Figure 6

Hypoxia activates the NF-κB P65 signaling pathway. (a, b) Western blot analysis of NF-κB P65 phosphorylation in C2C12 cells after CoCl₂ treatment for 8 hours (). (c, d) Immunofluorescence in C2C12 cells showing p-P65 upregulation with or without 2 hours of CoCl₂ treatment. Scale  μm. (e) Western blot analysis of nuclear and cytoplasmic protein expression of pyroptosis proteins at different time points after CoCl₂ treatment. The data are shown as the . , ; NS: no significant difference.

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