Research Article

AT1R-Mediated Apoptosis of Bone Marrow Mesenchymal Stem Cells Is Associated with mtROS Production and mtDNA Reduction

Figure 4

Mitochondrial ROS production and mitochondrial DNA leakage in BMMSCs after exposure to 0, 100 nM, 1 μM, and 10 μM Ang II for 12 hours. (a) MitoSOX™ superoxide indicator staining showing mitochondrial ROS levels in BMMSCs after exposure to different doses of Ang II. (b) PicoGreen staining showing mitochondrial DNA levels in BMMSCs after exposure to different doses of Ang II. (c) Quantification of MitoSOX fluorescence density (AU). (d) Quantification of PicoGreen fluorescence density except nuclei (AU). Bar graphs represent ( per group). vs. control.