Stimulation of Na⁺/K⁺-ATPase with an Antibody against Its 4^th Extracellular Region Attenuates Angiotensin II-Induced H9c2 Cardiomyocyte Hypertrophy via an AMPK/SIRT3/PPARγ Signaling Pathway

Figure 1

Effects of DR-Ab on Ang II-induced H9c2 cardiomyocyte hypertrophy. DR-Ab (2 μM) was given 30 min before treatment with Ang II (100 nM) for 48 h. (a, b) Representative immunofluorescence staining (a) and group data (b) showing that DR-Ab reversed enlarged cell size caused by Ang II. Red: α-actinin. Blue: DAPI. Scale bar, 25 μm. . (c) qRT-PCR analysis showing the mRNA levels of ANP, BNP, and β-MHC. . (d–g) DR-Ab reversed Ang II-induced loss of plasma membrane NKA α1 (e, f), increase of endosome NKA α1 (g), and downregulation of NKA activity (d). . Scale bar, 30 μm. Blue: DAPI. Green: NKA α1 staining. versus control group, † versus Ang II alone group.

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