Resveratrol Enhances Apoptotic and Oxidant Effects of Paclitaxel through TRPM2 Channel Activation in DBTRG Glioblastoma Cells

Figure 2

Effect of resveratrol (RESV and 50 μM for 24 hours) and paclitaxel (PAX and 50 μM for 24 hours) incubations on the Ca²⁺ florescence intensity through TRPM2 activation in the DBTRG cells. The cells were stained with Fluo3 calcium dye, and the of fluorescence in 15 mm² of cell as arbitrary unit are presented; independent experiments. The cells were extracellularly stimulated by cumene hydroperoxide (CPx and 1 mM), but they were extracellularly inhibited by ACA (0.025 mM). The samples were analyzed by a laser confocal microscope fitted with a 40x oil objective. ( versus control (Ctr) group. versus Ctr + CPx. versus Ctr + CPx + ACA group. versus RESV + PAX group. versus RESV + CPx and PAX + CPx groups. versus RESV + CPx + ACA and PAX + CPx + ACA groups. versus PAX + RESV group. versus PAX + RESV + CPx group).

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