(a)
(b)
(c)
Figure 5: Cell viability and Glo1 function in HUVECs. HUVECs were treated with 400 μM MG and glycine (0.5 mM-4 mM) for 72 h. (a) Cell viability determined by CCK-8. The experiment was performed in triplicate and repeated four times. (b) Intracellular GSH/GSSG ratio in HUVECs. The experiment was performed in triplicate and repeated three times. (c) The activity of Glo1 in HUVECs. The experiment was performed in triplicate and repeated four times. Control: cells incubated in MEM. The medium for all controls and experimental samples had a glucose level of 5.5 mM. MG: methylglyoxal. Gly: glycine. , , compared with the control group. , , compared with cells treated with 400 μM MG.