Acid Stress Triggers Resistance to Acetic Acid-Induced Regulated Cell Death through Hog1 Activation Which Requires RTG2 in Yeast

Figure 3

Hog1 phosphorylation in WT and ∆rtg2 mutants. (a) Cells were grown up to exponential phase (U), incubated at low pH, and (b) treated with acetic acid (AcOH). Cell protein extracts were prepared from WT and ∆rtg2 cells at the indicated times and analyzed by immunoblot with anti-P-Hog1p and anti-Hog1p antibodies. Cell extracts from ∆hog1 and ∆hog1∆rtg2 cells have been analyzed as a negative control. Ponceau staining was used as loading control. The histogram reports the ratio between phosphorylated (P-Hog1) and total Hog1 as quantified by densitometric analysis of the film normalized with Ponceau staining and expressed in arbitrary units. Reported values are the deviations of three independent experiments. Paired Student’s -test: statistically significantly different with and when comparing Δrtg2 versus WT under acid stress.

(a)

(b)