Propofol Alleviates Apoptosis Induced by Chronic High Glucose Exposure via Regulation of HIF-1α in H9c2 Cells
The HIF-1α silence mimicked the effect of propofol in H9c2 cells during high glucose stress. (a, b) Representative image of HIF-1α silence vesicles in H9c2 cells, which was, respectively, transfected with 50 nM siRNA-HIF-1α-1, siRNA-HIF-1α-2, or siRNA-HIF-1α-3. (c, d) Levels of Bcl-2 and cleaved caspase-3 in the high glucose-induced and siRNA-transfected H9c2 cells. (e) Western blotting of Bcl-2 and cleaved caspase-3 in H9c2 cells treated with 20 μM propofol or HIF-1α silence. (f) Levels of proinflammatory cytokines in H9c2 cells treated with 20 μM propofol or HIF-1α silence. Data were shown as from five independent experiments. ,, and versus control; #,##, and ### versus the high glucose-treated group without drugs. Model = high glucose model group; S1 = siRNA-HIF-1α-1 group; S2 = SiRNA-HIF-1α-2 group; S3 = SiRNA-HIF-1α-3 group; Ctrl = blank control group; 0 = high glucose model group; P20 = 20 μM propofol pretreatment group; SiRNA = HIF-1α silence group; and HG = 22 mM high glucose culture.
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