Research Article
FGF21 Mediates Mesenchymal Stem Cell Senescence via Regulation of Mitochondrial Dynamics
Figure 2
FGF21 mediates the replicative senescence of BM-MSCs. (a) Western blotting and quantitative analysis of the level of FGF21 protein in BM-MSCs at P4, P8, and P12 (i, ii). (b) Western blotting and quantitative analysis of the level of FGF21, p53, and p21 proteins in control siRNA or FGF21 siRNA-treated BM-MSCs at P4 (i, ii). (c) Representative images of SA-β-gal staining in control siRNA or FGF21 siRNA-treated BM-MSCs at P4 (i). Percentage of SA-β-gal-positive senescent cells in control siRNA or FGF21 siRNA-treated-BM-MSCs at P4 (ii). (d) Representative images of Ki-67 staining in control siRNA or FGF21 siRNA-treated BM-MSCs at P4 (i). Percentage of Ki-67-positive senescent cells in control siRNA or FGF21 siRNA-treated BM-MSCs at P4 (ii). Data are expressed as with per group. . Scale μm.
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