FGF21 Mediates Mesenchymal Stem Cell Senescence via Regulation of Mitochondrial Dynamics

Figure 4

Silencing FGF21 induces ROS generation via regulation of mitochondrial dynamics. (a) Representative images of MitoTracker staining in BM-MSCs at P4 and P12. (b) Western blotting and quantitative analysis of the levels of p-Drp1/Drp1 and Mfn2 proteins in BM-MSCs at P4 and P12 (i, ii). (c) Western blotting and quantitative analysis of the level of p-Drp1/Drp1 and Mfn2 proteins in control siRNA or FGF21 siRNA or FGF21 siRNA+Mfn2 siRNA-treated BM-MSCs at P4 (i, ii). (d) Representative images of Mito-Sox staining in BM-MSCs at P4 from the control siRNA or FGF21 siRNA or FGF21 siRNA+Mfn2 siRNA-treated group (i). Quantitative analysis of the ROS generation in control siRNA or FGF21 siRNA or FGF21 siRNA+Mfn2 siRNA-treated BM-MSCs at P4 (ii). (e) Representative images of SA-β-gal staining in BM-MSCs at P4 from the control siRNA or FGF21 siRNA or FGF21 siRNA+Mfn2 siRNA-treated group (i). Percentage of SA-β-gal-positive senescent cells in control siRNA or FGF21 siRNA or FGF21 siRNA+Mfn2 siRNA-treated BM-MSCs at P4 (ii). Data are expressed as with per group. . Scale  μm.

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