Research Article
Pasteurized Orange Juice Rich in Carotenoids Protects Caenorhabditis elegans against Oxidative Stress and β-Amyloid Toxicity through Direct and Indirect Mechanisms
Figure 1
Effect of pasteurized juice from ‘Bahia’ and ‘Cara Cara’ oranges on intracellular ROS accumulation in C. elegans. (a) Wild-type animals were treated with either 1, 2, 5, or 10% of ‘Bahia’ or ‘Cara Cara’ juice for approximately 48 h since L1. ROS production was measured using the dye H2DCFDA. Results are expressed as H2DCFDA fluorescence levels. compared to respective not treated (NT) control and comparing 2% ‘Bahia’ with 2% ‘Cara Cara’ juice by 2-way ANOVA. (b) Wild-type animals were treated with either 1, 2, 5, or 10% of ‘Bahia’ or ‘Cara Cara’ juices for approximately 48 h since L1 and then incubated on 10 mM TBHP for 1 hour to induce oxidative stress. Results are expressed as mean H2DCFDA fluorescence of values. compared to NT under standard conditions by a two-tailed Student’s -test, compared to respective NT control under stress and comparing ‘Bahia’ orange with ‘Cara Cara’ juice in each concentration by 2-way ANOVA. Percentage difference (%) between ‘Bahia’ and ‘Cara Cara’ juices are indicated for those with #statistically significance.
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