Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 3

Research Article

Melatonin and Docosahexaenoic Acid Decrease Proliferation of PNT1A Prostate Benign Cells via Modulation of Mitochondrial Bioenergetics and ROS Production

Figure 3

ROS determination. Production of (a) hydrogen peroxide (H2O2)—values show pmol of H2O2/min/106 cells and SEM; production of (b) superoxide anion (O2●−)—values show the fluorescence intensity (FI) per cell × 104. Legend of panels (a) and (b): C: control (vehicle incubation); DHA: incubation with 100 μM of DHA for 48 h; MLT: incubation with 1 μM of melatonin for 48 h; DM: coincubation with 100 μM of DHA and 1 μM of melatonin for 48 h. Statistical analysis: (A) different from C; (B) different from DHA; (C) different from MLT. was considered statistically different. Three independent experiments in triplicate were considered for statistical analysis of H2O2 production and at least 250 cells for O2●−. (c) Detection of O2●− production after MitoSOX incubation. Images captured with 40x objective with an inverted fluorescence microscope.

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