Melatonin and Docosahexaenoic Acid Decrease Proliferation of PNT1A Prostate Benign Cells via Modulation of Mitochondrial Bioenergetics and ROS Production
ROS determination. Production of (a) hydrogen peroxide (H2O2)—values show pmol of H2O2/min/106 cells and SEM; production of (b) superoxide anion (O2●−)—values show the fluorescence intensity (FI) per cell × 104. Legend of panels (a) and (b): C: control (vehicle incubation); DHA: incubation with 100 μM of DHA for 48 h; MLT: incubation with 1 μM of melatonin for 48 h; DM: coincubation with 100 μM of DHA and 1 μM of melatonin for 48 h. Statistical analysis: (A) different from C; (B) different from DHA; (C) different from MLT. was considered statistically different. Three independent experiments in triplicate were considered for statistical analysis of H2O2 production and at least 250 cells for O2●−. (c) Detection of O2●− production after MitoSOX incubation. Images captured with 40x objective with an inverted fluorescence microscope.
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