Research Article

Patients with Incompetent Valves in Chronic Venous Insufficiency Show Increased Systematic Lipid Peroxidation and Cellular Oxidative Stress Markers

Table 2

Primary and secondary antibodies used in the immunohistochemical studies performed, showing the dilutions used and the specificities in their protocol.

AntigenSpeciesDilutionProviderProtocol specifications

eNOSRabbit1:100Abcam (ab66127)Citrate tampon in heat ()
iNOSRabbit1:500Abcam (ab95866)
NOX1Rabbit1:250Abcam (ab78016)EDTA () before incubation with blocking solution
NOX2Goat1:500Abcam (ab111175)
Anti-rabbit IgGMouse1:1000RG-96 (Sigma-Aldrich)
Anti-goat IgGMouse1:100A5420 (Sigma-Aldrich)