Research Article

Cell Type- and Exposure-Specific Modulation of CD63/CD81-Positive and Tissue Factor-Positive Extracellular Vesicle Release in response to Respiratory Toxicants

Figure 2

Effect of NAC on the EV release and cell metabolic activity during CSE exposure. Cells were exposed to CSE in the presence or absence of the thiol antioxidant NAC, and different readouts were performed. Concentration of (a) CD63+CD81+ EVs () and (b) CD63/CD81/CD9+TF+ EVs () as determined by bead-based flow cytometry. (c) Cell metabolic activity as determined by the MTT assay (). The box and whisker plots show the median (line in the box), 25th and 75th percentiles (outer lines of the box), and minimal and maximal values (whiskers). compared to the unexposed control (100%); # for CSE alone compared to CSE with NAC. Cell lines: BEAS-2B: bronchial epithelial cells; HEL-299 and MRC-5: pulmonary fibroblast cell lines; THP-1: monocyte-derived macrophages; HPMEC: human pulmonary microvascular endothelial cell line.
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