Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 1

Research Article

Salidroside Delays Cellular Senescence by Stimulating Mitochondrial Biogenesis Partly through a miR-22/SIRT-1 Pathway

Figure 1

Salidroside (SAL) delays the phenotypes of replicative senescence of human fibroblast 2BS cells. (a) SA-β-gal staining of 2BS fibroblasts grown from 30PD in DMEM supplemented with 10 μM salidroside (SAL). Control cells at 55PD became replicative senescence. Cells at 30PD were set as young control. Cells of a nonconfluent state were washed with PBS, fixed with 3% formaldehyde, and submitted for SA-β-gal staining. Cells were microphotographed at a magnification of . (b, c) Effect of SAL and resveratrol (RES) on the ROS level in near-senescent 50PD 2BS cells. Cells treated by SAL (10 μM) or RES (10 μM) for 24 h were stained by H2DCFDA for detection of intracellular ROS production. (b) Images of cells stained by H2DCFDA were obtained by using fluorescence microscopy. (c) Quantitative measurement of an intracellular ROS level was performed by flow cytometric analysis. ## versus 30PD control (Ctrl); versus control (Ctrl) at 55PD or 50PD.

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