Salidroside Delays Cellular Senescence by Stimulating Mitochondrial Biogenesis Partly through a miR-22/SIRT-1 Pathway

Table 1

The effect of salidroside on the life spans of 2BS cells in CPDs.

Group	Treatment	Time of transfer to a special medium		CPDs	Average PDs per week
I	Control	—	3
II	SAL (5 μM)	30PD	3
III	SAL (10 μM)	30PD	3

Note. Human fibroblast 2BS cells at 30PD were cultured in DMEM supplemented with salidroside (SAL) at 5 μM and 10 μM, respectively. When the confluence of the culture was reached 70-80%, the cells were split in ratios of 1 : 2 or 1 : 4 and were continually cultured in the indicated medium. Control cells were cultured in a 0.01% supplemented medium. CPDs (cumulative population doublings) were calculated as log2 (), where and were defined as the density of cells at the time of harvesting and seeding, respectively. The last culture was defined as the subculture that could not be confluent in 15 days. Data were obtained from three independent experiments ( vs. ).