ROS-Induced GATA4 and GATA6 Downregulation Inhibits StAR Expression in LPS-Treated Porcine Granulosa-Lutein Cells

Figure 9

Schematic diagram of the proposed molecular mechanism for LPS-induced downregulation of StAR expression and progesterone production in pGL. Treatment with LPS leads to the elevation of ROS and subsequently reduces GATA4 and GATA6 expressions, which finally reduced the expression of StAR. Elimination of ROS with melatonin or Vc could rescue the expressions of GATA4, GATA6, and StAR. LPS treatment also downregulates P450scc (encoded by the CYP11A1 gene) and 3β-HSD expressions, but not through the ROS/GATA4/GATA6 signaling pathway.