Neuroglobin Expression Models as a Tool to Study Its Function

Figure 2

Overview of the different types of Ngb expression models. The in vivo models comprise animal models of modulated Ngb expression (Ngb_Mod) on the one hand (i.e., Ngb-overexpressing and Ngb-deficient mouse models) and WT animals on the other hand. Ngb overexpression in WT animals can be obtained by injection of Ngb-expressing vectors or protein delivery. Ngb knockdown can be established by injection of anti-Ngb RNAs. The in vitro models comprise primary cells derived of the Ngb-modulated mouse models, primary cells from WT animals, and other cell types. To create Ngb overexpression or Ngb deficiency, similar techniques can be used as with the in vivo models.