Research Article

Tetramethylpyrazine Attenuates the Endotheliotoxicity and the Mitochondrial Dysfunction by Doxorubicin via 14-3-3γ/Bcl-2

Figure 5

TMP-treated alters the expression, phosphorylation, or sublocalization, or metabolism of the related active proteins in HUVECs of Dox injury. (a) Western blot’s banding of the related proteins in HUVECs. (b) Histogram of 14-3-3γ expression in the cytoplasm. (c) Histogram of p-Bad expression in the cytoplasm. (d) Histogram of Bcl-2 expression in the mitochondria. (e) Histogram of p-eNOS/eNOS expression in the cytoplasm. (f) The contents of NO in culture medium. On (a), from left to right, lane 1: control; lane 2: Dox; lane 3: TMP + Dox; lane 4: TMP + Dox + pAD/14-3-3γ-shRNA; lane 5: TMP + Dox + ABT-737. Data are presented as the mean ± SEM. for eight individual experiments. a: P < 0.01, versus control group; b: P < 0.01, versus Dox group; c: P < 0.01, versus TMP + Dox group.
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