Antistress Effects of San-Huang-Xie-Xin Decoction on Restraint-Stressed Mice Revealed by 1H NMR-Based Metabolomics and Biochemistry Analysis
Table 1
Potential biomarkers in mouse serum and their variations among groups.
No.
Metabolite
Chemical shift (ppm)a
Assignment
Control vs. normalb
SHXXD vs. controlb
1
LDL/VLDL
0.87 (br), 1.27 (br)
–CH3, –CH2
↑
—
2
Lactate
1.32 (d), 4.11 (q)
β–CH3, α–CH
↑
↓
3
Alanine
1.47 (d)
β–CH3
↑
↓
4
Lipids
1.57 (br)
–CH2–CH2–C=O
↑
↓
5
N-acetyl glycoproteins
2.04 (s)
–CH3
↑
↓
6
Glycerol
3.56 (m), 3.69 (m)
–CH2, –CH2
↑
↓
7
Choline
3.20 (s)
–N–(CH3)3
↑
↓
8
Phosphorylcholine
3.22 (s)
–N–(CH3)3
↑
—
9
Acetoacetate
2.23 (s)
–CH3
↓
↑
10
Valine
0.98 (d), 1.03 (d)
γ–CH3, γ–CH3
↓
↑
as: singlet; d: doublet; q: quartet; m: multiplet; br: broad resonance. bMetabolites with “↑/↓” means increased/decreased; “—” means no significant differences. ,, and indicate ,, and , respectively.