Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 4

Research Article

Resveratrol Improves Boar Sperm Quality via 5AMP-Activated Protein Kinase Activation during Cryopreservation

Figure 4

Location of AMPK in postthaw boar sperm was analyzed by immunofluorescence (a). Effects of different concentrations of resveratrol on postthaw boar sperm AMPK phosphorylation (b–d). (b) Western blotting image is showing the expression of the p-AMPK, AMPK, and α-tubulin of postthaw boar sperm. (c, d) Quantitative expression of the p-AMPK and AMPK over α-tubulin generated from western blotting (b). (e) Western blotting image is showing the expression of the p-AMPK, AMPK, and α-tubulin of sperm in the H2O2-induced oxidative stress model. (f, g) Quantitative expression of the p-AMPK and AMPK over α-tubulin generated from western blotting (e). Data are the ( independent replicates). Columns with different uppercase letters differ significantly ().
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