Research Article

HIV-1 Reverse Transcriptase Promotes Tumor Growth and Metastasis Formation via ROS-Dependent Upregulation of Twist

Figure 6

Relative levels of expression of Twist by the derivatives of 4T1luc2 cells expressing variants of consensus HIV-1 FSU_A reverse transcriptase (Table 2) after 14 days in culture (a) and correlation of the relative expression of Twist with the levels of expression of RT_A variants (b). Expression level of Twist was normalized to the expression of HPRT1 and calculated as fold change compared to the levels exhibited by the parental 4T1luc2 cells. Values are presented as from the assay run in triplicates. The amount of RT_A variant produced per cell and expression of Twist at day 14 were correlated using Spearman ranking test (, ); .
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(b)