Bucindolol Modulates Cardiac Remodeling by Attenuating Oxidative Stress in H9c2 Cardiac Cells Exposed to Norepinephrine

Figure 2

Active caspase 3/7 was determined in H9c2 cardiac cells incubated with norepinephrine (10 μM) during a period of 24 h, followed by 8 h of bucindolol (10 μM) treatment (c). Representative dot plots indicating the cell populations of all groups (a). Representative histogram showing FL1-H fluorescence versus cell counts, indicating activated caspase-3/7 (M2 area) (b). Values expressed as from 3 independent experiments. Two-way ANOVA followed by Tukey: ^A vs. Control Untreated (CTL), ^B vs. Norepinephrine Untreated (NE), ⁺ CTL and Control+Bucindolol (CTL+BCD) vs. NE and Norepinephrine+Bucindolol (NE+BCD), and CTL+BCD and NE+BCD vs. CTL and NE.

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(b)

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