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Figure 4: Effect of BA6 on the changes of the metabolizing enzymes of reactive oxygen species (ROS) and energy metabolism in A549 cells. (a) A549 cells were treated with various concentrations of BA6 for 24 h. Cell lysates were loaded for Western blot analysis using various antibodies, including Cu/Zn SOD, MnSOD, catalase, and β-actin antibodies. β-Actin was detected as a normalization internal control. The Cu/Zn SOD (b), MnSOD (c), and catalase (d) protein levels were quantified by ImageJ software and normalized with that of the β-actin level. (e) Western blot showed the changes of the protein level of ATP5A in BA6-treated A549 cells. The β-actin level was used as the loading control. (f) The ATP5A protein band was quantified by ImageJ software and normalized with that of the β-actin level. (g) The effects of various doses of BA6 treatment for 24 h on the total ATP production. The values were calculated as from three independent experiments. Significance was determined by -test: and as compared with untreated (0 μM) cells.