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Figure 6: Effect of MitoTEMPO on the reverse of BA6-induced cellular cytotoxicity, mtROS production, and MMP disruption in the A549 cell. (a) After prior incubation with MitoTEMPO (10 μM) for 4 h, A549 cells were treated without or with BA6 (10 μM) for an additional 24 h and photographed by phase contrast microscopy at 200x magnification. (b) The proliferation of A549 cells was determined by the MTT assay, which is performed to measure cell viability. Cell viability (%) is expressed as a percentage, as compared to the untreated cells (0 μM). The results are expressed as the () of three independent experiments. (c) The representative flow cytometry histogram demonstrates the effects of the MitoTEMPO on MitoSOX Red fluorescence expressions in A549 cells treated without or with BA6 (10 μM) for 24 h. (d) Quantification of MitoTEMPO reversed the BA6-induced production of mtROS percentages by flow cytometry histogram. (e) The representative flow cytometry analysis demonstrates the effects of MitoTEMPO on rhodamine 123 fluorescence expressions in A549 cells treated without or with BA6 (10 μM). (f) Quantification of inhibitory effects of MitoTEMPO on BA6-induced MMP disruption analyzed by rhodamine 123 and flow cytometry. Values are the of three independent experiments. Significance was determined by Student’s -test: and compared with untreated cells; as compared with the BA6 only group.