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Figure 7: MitoTEMPO inhibited BA6-induced apoptosis in A549 cells. Pretreatment was conducted with MitoTEMPO (10 μM) for 4 h, and then, A549 cells were treated without or with BA6 (10 μM) for an additional 24 h. (a) This dot plot diagram was divided into four quadrants to present the ratio of apoptotic cells by using a flow cytometer. The representative flow cytometry analysis demonstrated the effects of MitoTEMPO on annexin V/PI staining expressions in A549 cells treated without or with BA6 (10 μM). (b) The statistics show that MitoTEMPO is capable of reversing BA6-induced apoptosis (annexin V/PI double-positive and annexin V+/PI- staining). (c) The representative Western blot analysis demonstrated the effects of the MitoTEMPO on caspase-9 and caspase-3 protein expressions in A549 cells treated without or with BA6 (10 μM). Here, β-actin was detected as the internal control. The procaspase-9 and cleaved caspase-9 (d) and procaspase-3 and cleaved caspase-3 (e) protein levels were quantified by ImageJ software, normalized with that of the β-actin level, and expressed as a normalization of the control. Values are the of three independent experiments. Significance was determined by -test: as compared with untreated cells; as compared with the BA6 only group.