Research Article

TRPA1 Promotes Cardiac Myofibroblast Transdifferentiation after Myocardial Infarction Injury via the Calcineurin-NFAT-DYRK1A Signaling Pathway

Figure 2

TRPA1-mediated CMF activation in the fibrotic response to MI injury. (a and b) Western blot analysis and quantification of TRPA1 protein expression from adult CFs isolated from the ventricles of sham or MI-injured mice. Molecular weights in kDa are shown to the right of the blots. per group. (c and d) Immunofluorescence staining and quantification of CMF numbers (white arrows) in the cardiac infarction border zone 7 days after injury. Myofibroblasts are positive for α-SMA (red) and negative for CD-31 (green) (scale  μm). Five sections were measured per heart, per group. (e and f) Western blot analysis and quantification of Postn protein expression in adult CFs isolated from the ventricles of sham or MI-injured mice. Molecular weights in kDa are shown to the right of the blots. per group. The error bars represent the and . The data are representative of the results of three or more independent experiments.
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