MHY2233 Attenuates Replicative Cellular Senescence in Human Endothelial Progenitor Cells via SIRT1 Signaling

Figure 2

SIRT1 deacetylase activity of MHY2233: (a) schematic diagram to show isolation of EPCs from the human umbilical cord blood, treatment with drugs, and other basic experimental designs. (b) Chemical structure of MHY2233. (c) EPCs were seeded in 96-well plates and treated with different concentrations of MHY2233 for 24 h ( per group). Cytotoxicity assay of MHY2233 at different concentrations was determined using WST-8 assay ( per group). (d) Relative mRNA levels of SIRT1 to SIRT7 in young and senescent EPCs were evaluated by qRT-PCR ( per group). (e) Relative mRNA expression of SIRT1 to SIRT7 in senescent EPCs treated with DMSO and MHY2233 ( per group). (f) SIRT1 deacetylase activity was performed using a commercially available kit. Measurement of SIRT1 deacetylase activity of MHY2233 along with positive and negative controls ( per group). , , and vs. DMSO (control) and indicated groups by a one-way ANOVA test.

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