Research Article

H2S Protects against Cardiac Cell Hypertrophy through Regulation of Selenoproteins

Figure 3

H2S or H2Se reverses H2O2-induced cell hypertrophy. H9C2 cells were pretreated with 30 μM NaHS or 0.3 μM Na2Se for 30 minutes, then incubated with 200 μM H2O2 for additional 24 hours followed by staining with 1 ng/ml WGA coupled with a fluorophore for 45 minutes. Visualization of cell size (a) was observed with a fluorescence microscope, and the cell surface area was measured with ImageJ software (b). vs. control; # vs. H2O2 treatment. . (c, d) Induced mRNA expressions of ANF and BNP by H2O2 treatment. mRNA expression was analyzed by real-time PCR. vs. control. .