UFL1 Alleviates Lipopolysaccharide-Induced Cell Damage and Inflammation via Regulation of the TLR4/NF-κB Pathway in Bovine Mammary Epithelial Cells

Figure 1

UFL1 is highly expressed in LPS-induced bovine mammary gland tissue and BMECs. (a) Bovine mammary glands were analyzed by immunohistochemistry. (b) Immunofluorescent staining of BMECs with anti-UFL1 antibody. (c) Representative TUNEL staining of normal and mastitis bovine mammary gland. (d) Representative H&E staining of normal and mastitis bovine mammary gland. (e) Expression of UFL1mRNA in bovine mammary gland tissue and BMECs treated with LPS. (f) Expression of UFL1 protein in bovine mammary gland tissue and BMECs treated with LPS. (g) Effect of UFL1 siRNA on the expression of UFL1 in BMECs. (h) UFL1 expression after BMECs was transfected with UFL1 overexpression plasmid. (i) Immunofluorescent staining of BMECs with anti-UFL1 antibody in siRNA-treated cells stimulated with LPS. (j) Immunofluorescent staining of BMECs with anti-UFL1 antibody in UFL1 overexpression plasmid-treated cells stimulated with LPS. Data are presented as the of the mean (SEM) of three independent experiments. .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)